Importance of biological replicates in biological research
Tania, a PhD student is conducting experiment on chickpea plants to check a specific gene expression level upon providing phosphorous deficiency stress. She is repeating this experiment for the third time and she could not reach to a conclusion yet, as her data shows specific pattern of gene expression difference among the control and stressed (she got higher expression in the stressed plants) but there is no statistical significance in this difference which is making her data scientifically invalid. Most of the researcher may have faced similar kind of problem during experiment. This happens mostly when the sample size or sample number is low due to low number of biological replicates, which is also called experimental replicates. The number of sample sets you are taking during an experiment is called replicates, which indicates for how many samples you are conducting the same experiment? In biological research, these replicates are called 'biological replicates'. For th...